The knowledge of medical ninjutsu can be used for a variety of purposes apart from pure healing, such as creating and treating poisons as seen with Shizune who uses her extensive medical knowledge to create a poisonous gas, performing autopsies or surgeries, or attacking a person's body directly, deranging the target's nervous system or sending them into a comatose state by overriding their body with chakra. Medical Ninjutsu in itself can also be used offensively, witnessed in Kabuto Yakushi's use of chakra scalpels in battle, overriding his opponents body with medical chakra and Tsunade has also demonstrated the ability to use medical ninjutsu offensively as shown when she attempted to use medical ninjutsu to kill Orochimaru. Tsunade, renowned as the greatest medical-nin in the world, was able to apply the extreme chakra control required for medical ninjutsu to create chakra-enhanced strength, an ability she taught to her student, Sakura Haruno.
- The Creation Rebirth technique is considered to be the absolute pinnacle of all medical ninjutsu, and the ultimate regeneration technique.
- Hashirama Senju could use medical ninjutsu with such proficiency that he could heal himself without the need of forming hand seals, a feat initially believed to be impossible even for Tsunade, though she's able to replicate it through the use of her Strength of a Hundred Technique.